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| Status |
Public on Dec 18, 2020 |
| Title |
Genome-wide maps of nucleolus interactions in human cells using 4xAP3 DamID |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
We used DamID to map nucleolus interactions in human cells using a 4x tandem repeat of the nucleolar targeting sequence of AP3D1 (Scott, 2010) ("4xAP3"). These interactions are mapped in various human cell lines, including human K562 cells
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| Overall design |
A DamID expression vector was created where Dam was fused to the 4xAP3 sequence described above to target to the nucleolus. Wild-type K562 cells were transduced with lentiviral constructs in more replicates, Dam-4xAP3 or Dam only and collected by three days after transduction for quantification of genome-wide methylation levels via next-generation sequencing.
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| Contributor(s) |
van Schaik T, Peric-Hupkes D, van Steensel B |
| Citation missing |
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| |
| Submission date |
Apr 14, 2020 |
| Last update date |
Dec 21, 2020 |
| Contact name |
Bas van Steensel |
| E-mail(s) |
b.v.steensel@nki.nl
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| Phone |
+ 31 20 512 2040
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| Fax |
+31 20 669 1383
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| URL |
http://www.nki.nl/nkidep/vansteensel
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| Organization name |
Netherlands Cancer Institute
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| Department |
division of Molecular Biology
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| Lab |
van Steensel group
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| Street address |
Plesmanlaan 121
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| City |
Amsterdam |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA625198 |
| SRA |
SRP256313 |
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