 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 18, 2020 |
| Title |
Endothelial cells control muscle regeneration through angiocrine lactate |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We studied metabolic angiocrine mechanisms by which endothelial cell_ECs_ can contribute to muscle regeneration from ischemia by using endothelial specific pfkfb3 knockout mice_pfkfb3DEC_ after hind-limb ischemia_HLI_. During muscle regeneration, monocytes are recruited to the injured area and rapidly become macrophages which initially exhibit a more pro-inflammatory M1-like phenotype but soon thereafter functionally repolarize towards an M2-like phenotype to actively support muscle regeneration. Interestingly, macrophages derived from pfkfb3DEC failed to polarized to M2-like macrophages after HLI. Reduced macrophage polarization impairs angiogenesis and muscle regeneration. The RNAseq data are pfkfb3DEC and pfkfb3WT muscle derived macrophages 3 days after HLI.
|
| |
|
| Overall design |
pfkfb3EC and pfkfb3WT muscle derived macrophages 3 days after HLI.
|
| |
|
| Contributor(s) |
Zhang J, Muri J, Fitzgerald G, Gorski T, Gianni-Barrera R, Masschelein E, D'Hulst G, Gilardoni P, Turiel G, Fan Z, Wang T, Planque M, Carmeliet P, Pellerin L, Wolfrum C, Fendt S, Banfi A, Stockmann C, Soro-Arnaiz I, Kopf M, De Bock K |
| Citation(s) |
32492393 |
| |
| Submission date |
Apr 13, 2020 |
| Last update date |
Jun 16, 2020 |
| Contact name |
jing zhang |
| Organization name |
DHEST
|
| Department |
Department Health Sciences and Technology
|
| Lab |
Laboratory of Exercise and Health
|
| Street address |
schorenstrasse 16
|
| City |
schwerzenbach |
| State/province |
Schwerzenbach |
| ZIP/Postal code |
8603 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA625078 |
| SRA |
SRP256293 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE148584_macrophage_seq-list.xlsx |
2.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...