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| Status |
Public on Apr 03, 2023 |
| Title |
TBBPA, TBBPS and TCBPA disrupt hESCs hepatic differentiation and promote the proliferation of differentiated cells seemingly via up-regulating the FGF10 signaling pathway |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Halogenated flame retardants (HFRs) Tetrabromobisphenol A (TBBPA), Tetrabromobisphenol S (TBBPS) and Tetrachlorobisphenol (TCBPA) are wildly applied in manufacturing industry to improve the fire safety of the products. Recent reports demonstrated that TBBPA, TBBPS and TCBPA can be detected in pregnant women serum at nanomolar-level. Considering TBBPA have also been detected in the cord serum and the structural similarity of the 3 HFRs, it is necessary to pay attention to their development toxicity. Liver is the important detoxic organ but also target of TBBPA since it has been demonstrated that TBBPA exposure lead to increased liver weight in mouse and rat. So the developmental hepatic toxicity of the 3 HFRs were studied in this current research with the transcriptomic and human embryonic stem cells hepatic differentiation based toxicity evaluation system. A hepatic differentiation specific lineage development map and cell lineage - gene expression annotations were made and used for easily assessing the lineage alternations caused by toxicant treatment. Together with GO and KEGG analysis, this study mainly demonstrated that, the 3 HFRs have many common disruptive effect on hepatic differentiation, while only TCBPA significantly inhibited hepatic differentiation. The up-regulation of genes related to cell cycle and FGF10 signaling pathway at relatively late stage of hepatic differentiation indicates the proliferation of hepatoblasts were promoted by the 3 HFRs, likely via up-regulating the FGF10 signaling pathway. The proliferating promoting effect may partly explain why liver gain weight in rodents exposed to TBBPA.
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| Overall design |
A total of 24 samples analyzed, with 2 H9 samples here, 16 samples in our unpublished research (GSM3712591-GSM3712606 in GSE129412 and GSM4473072-GSM4473087 here), and 6 samples (GSM3518767-GSM3518772) from our previously published research.
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| Contributor(s) |
Yang R, Liang S, Liu S, Liang X, Jiang L, Yin N, Faiola F |
| Citation(s) |
32653787 |
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| Submission date |
Apr 12, 2020 |
| Last update date |
Jul 03, 2023 |
| Contact name |
Renjun Yang |
| E-mail(s) |
rjyang@rcees.ac.cn
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| Phone |
18811781909
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| Organization name |
RCEES
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| Street address |
双清路18号林业大学北门
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| City |
北京市 |
| State/province |
北京 |
| ZIP/Postal code |
100085 |
| Country |
China |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA624865 |
| SRA |
SRP256239 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE148518_Expected_count_of_genes.txt.gz |
773.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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