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Series GSE148506 Query DataSets for GSE148506
Status Public on Jul 10, 2020
Title Single-cell profile of atrial human fibroblasts in cardiac arrhythmia atrial fibrillation (AF) - SmartSeq2
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Atrial fibrillation (AF), the most common cardiac arrhythmia, is a major contributor to population mortality and morbidity. The goal of this study is to explore molecular mechanisms of the AF-induced profibrotic remodelling in human atrial fibroblasts (ACFs). Specifically, we assessed single-cell transcriptome of cultured human ACFs treated with calcitonin(CT) or vehicle (from 6 individual patients) and of freshly-isolated ACFs from patients with AF (4 individual patients) vs controls (4 individual patients) (SR). We found that ACF transcriptome was unaltered by CT in cultured ACFs, and identified 5 transcriptional clusters with 23 differentially expressed transcripts in AF in freshly-ioslated ACFs.
 
Overall design mRNA profile of cultured (6 biological replicates conducted in single technical replicates) and freshly-isolated (8 biological replicates conducted in single technical replicates) human ACFs.
 
Contributor(s) Reilly S, Antanaviciute A
Citation(s) 33149301
Submission date Apr 11, 2020
Last update date Nov 20, 2020
Contact name Svetlana Reilly
Organization name University of Oxford
Department Cardiovascular Medicine, Radcliffe Dept of Medicine
Street address JOhn Radcliffe Hospita, Headley Way
City Oxford
State/province Oxfordshire
ZIP/Postal code OX9 3DU
Country United Kingdom
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (384)
GSM4472581 Plate1_Well_A1, AF, ACFs
GSM4472582 Plate1_Well_A10, SR, ACFs
GSM4472583 Plate1_Well_A11, SR, ACFs
This SubSeries is part of SuperSeries:
GSE148507 Single-cell profile of atrial human fibroblasts in cardiac arrhythmia atrial fibrillation
Relations
BioProject PRJNA624784
SRA SRP256228

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148506_raw_reads_counts_matrix.txt.gz 9.3 Mb (ftp)(http) TXT
GSE148506_well_meta_data.txt.gz 31.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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