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| Status |
Public on Jun 25, 2009 |
| Title |
Comparative genomics using microarrays studied in Metarhizium anisopliae 2575 and 324 |
| Organism |
Metarhizium anisopliae |
| Experiment type |
Genome variation profiling by array
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| Summary |
The entomopathogen Metarhizium anisopliae contains strains with wide host ranges and specialist strains adapted to particular hosts. Patterns of gene duplication, divergence and deletion in three generalist and three specialist strains were investigated by heterologous hybridization of genomic DNA to genes from the generalist strain ARSEF 2575. Many sequences from 2575 that are highly conserved in fungi showed rapid evolution and loss in specialist Metarhizium genomes. Some poorly hybridizing genes in specialists were functionally coordinated, including several involved in toxin biosyntheses and sugar metabolism in root exudates, indicative of reductive evolution. This suggests that specialists are loosing genes required to live in alternative hosts or as saprophytes. Several components of mobile genetic elements were also highly divergent or lost in specialists. Exceptionally, the genome of the specialist strain ARSEF 443 contained extra insertion elements that might play a role in generating evolutionary novelty.
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| Overall design |
Three microarray slides were used in comparison (cDNAs are replicated in triplicate on each slide). 324 strain’s DNA was co-hybridized with strain ARSEF 2575 DNA in dye swapping replicate experiments and the relative hybridization efficiency (fluorescence ratio) of their DNA for strain ARSEF 2575 genes was compared. This array harbors PCR amplified fragments from the unique cDNA clones from M. anisopliae var. anisopliae ARSEF 2575 and a few genes from M. anisopliae var. acridum ARSEF 324 absent from the libraries of ARSEF 2575. In total, 1730 amplified clones were printed in triplicates on the slides. Additional background control was provided by 30 randomly distributed spots of 3×SSC buffer. Printing, hybridization, and scanning of slides was as described before (Freimoser et al., 2005).
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| Contributor(s) |
Wang S, Raymond S |
| Citation(s) |
19395664 |
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| Submission date |
Feb 16, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Sibao Wang |
| E-mail(s) |
swang1@umd.edu
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| Phone |
301-405-1603
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| Fax |
3013149290
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| Organization name |
University of Maryland
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| Department |
Entomology
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| Lab |
Insect pathology
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| Street address |
Baltimore Avenue
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| City |
College Park |
| State/province |
MD |
| ZIP/Postal code |
20742 |
| Country |
USA |
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| Platforms (1) |
| GPL8193 |
UMD Metarhizium anisopliae V1.0 |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA112121 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14846_RAW.tar |
170.0 Kb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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