Expression profiling by high throughput sequencing
Summary
3’RACE-seq experiments were conducted to determine the impact of URT1 ectopic expression on the poly(A) tail length profile of both a GFP reporter mRNA co-expressed in Nicotiana benthamiana leaves and an endogenous mRNA encoding PATHOGENESIS-RELATED PROTEIN 2 (PR2)
Overall design
3’RACE-seq method was used to measure poly(A) tail sizes and analyze uridylation level. This protocol is based on the ligation of a primer at the 3’ end of RNAs, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.
Molecular connection between the TUTase URT1 and decapping activators. Hélène Scheer, Caroline de Almeida, Emilie Ferrier, Laure Poirier, David Pflieger, François M Sement, Sandrine Koechler, Christina Piermaria, Johana Chicher, Lauriane Kuhn, Philippe Hammann, Hélène Zuber, Dominique Gagliardi. bioRxiv 2020.05.26.114322.
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