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| Status |
Public on Mar 27, 2020 |
| Title |
Diurnal patterns of gene expression in the dorsal vagal complex and the central nucleus of the amygdala |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Genes that establish the circadian clock have differential expression with respect to solar time in central and peripheral tissues. Here, we find circadian-time-induced differential expression in a large number of genes not associated with circadian rhythms in two brain regions lacking overt circadian function: the dorsal vagal complex (DVC) and the central nucleus of the amygdala (CeA). These regions primarily engage in autonomic, homeostatic, and emotional regulation. However, we find striking diurnal shifts in gene expression in these regions of male Sprague Dawley rats with no obvious patterns that could be attributed to function or region. These findings have implications for the design of gene expression studies as well as for the potential effects of xenobiotics on these regions that regulate autonomic and emotional states.
Micropunches of dorsal vagal complex (DVC) and central nucleus of the amygdala (CeA) were collected from male, Sprague Dawley rats at three different times of the day. Animals were not experimentally different in any way except for the time of sacrifice and collction. Gene expression of 145 genes were measured with Fluidigm's Biomark 96.96 platform.
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| Overall design |
High-throughput qRT-PCR profiling of 145 genes using Fluidigm's 96.96 array. Bilateral samples from a single animal were considered as one sample. There was no inter-animal pooling of samples. Samples were collected at ZT3, 5, and 9 (where ZT0 is lights on in the facility).
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| Contributor(s) |
Staehle MM, O'Sullivan S, Vadigepalli R, Kernan K, Gonye GE, Ogunnaike BA, Scwhaber JS |
| Citation(s) |
32477043 |
| |
| Submission date |
Mar 26, 2020 |
| Last update date |
Jun 08, 2020 |
| Contact name |
Mary Staehle |
| E-mail(s) |
staehle@rowan.edu
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| Organization name |
Rowan University
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| Department |
Biomedical Engineering
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| Street address |
201 Mullica Hill Road
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| City |
Glassboro |
| State/province |
NJ |
| ZIP/Postal code |
08028 |
| Country |
USA |
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| Platforms (1) |
| GPL28311 |
DanielBaughInstitute-BioMark145 |
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| Samples (72)
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| Relations |
| BioProject |
PRJNA615538 |
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