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| Status |
Public on Aug 31, 2020 |
| Title |
Gene expression in human embryonic stem cells (hESCs) upon the differentiation to neural lineages by timed delivery of retinoic acid |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report gene-expression profiling of hESCs differentiating to neuronal lineages upon the treatment with Retinoic Acid and Smoothened Agonisnt. We study weather RA patterning activity relies on duration of RA exposure and can be used to direct differentiation of hESCs into distinct neuronal cell types.
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| Overall design |
Different time points of hESCs differentiation (day 0, 2, 9, 12, 14, 21): 20 samples resenting 8 conditions in 2 biological replicates.
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| Contributor(s) |
Ericson J, Alekseenko Z |
| Citation(s) |
35650213 |
| |
| Submission date |
Mar 23, 2020 |
| Last update date |
Jun 17, 2022 |
| Contact name |
Johan Ericson |
| E-mail(s) |
johan.ericson@ki.se
|
| Organization name |
Karolinska Instituet
|
| Department |
Cell and Molecular Biology
|
| Lab |
Johan Ericson's Group
|
| Street address |
Solnavägen 9
|
| City |
Stockholm |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA614495 |
| SRA |
SRP253722 |
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