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| Status |
Public on Jun 30, 2020 |
| Title |
Gene expression data for human intervertebral discs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The human intervertebral disc (IVD) is a complex and dynamic structure that functions to provide spinal stability, mobility and flexibility. It comprises three main compartments: 1) a water-rich central compartment called the nucleus pulposus (NP), which is enveloped by 2) the annulus fibrosus (AF) and sandwiched between 3) two cartilaginous endplates (EP) from which the IVD gains its nutrition and provides a means to get rid of metabolic waste.
The IVD is constantly under extreme conditions of hypoxia, nutrition, and loading stresses. As such, it wears with ageing, and the rates of wearing may be sped up by a multitude of risk factors. Phenotypically, the AF may become weaker that a portion of it may protrude into neighboring tissues, resulting in disc bulging, or even worse, it may rupture and form a herniation
Most striking of all, magnetic nuclear imaging (MRI) of the NP may become darker, with spotted high-intensity zones (HIZs), indications of dehydration and annular disruptions (Peng, et al. 2006). In a herniated or even collapsed disc, the contents of NP may leak into the neighboring spaces and external cells (such as macrophages) may infiltrate into the NP (Nakazawa, et al. 2018), causing the disc functions to be partially or fully compromised. The underlying mechanism of these involves complex interplays of cellular and molecular changes and remains poorly understood; nevertheless, the process is suggested to be triggered and driven by both environmental and genetic factors, or their combinations.
In an effort to study the molecular dynamics of the ageing process of the human IVD, we profiled the transcriptomes of two groups of individuals: a young and non-degenerated group (N=2) and an aged and degenerated group (N=2). We profiled the transcriptomes of both AF and NP for each individual.
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| Overall design |
We measured the transcriptome of eight samples, corresponding to 4 individuals' AF and NP structures, respectively.
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| Contributor(s) |
Yee A, Tam V, Chen P, Chan D |
| Citation(s) |
33382035 |
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| Submission date |
Mar 23, 2020 |
| Last update date |
Jan 01, 2021 |
| Contact name |
Peikai Chen |
| E-mail(s) |
pkchen1@hku.hk
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| Phone |
852-258315217
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| Organization name |
The University of Hong Kong
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| Department |
Faculty of Medicine
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| Street address |
Sassoon Road 5
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| City |
Pok Fu Lam |
| ZIP/Postal code |
10000 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA614489 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE147383_HG-U133_Plus_2.na28.annot.csv.gz |
21.5 Mb |
(ftp)(http) |
CSV |
| GSE147383_RAW.tar |
43.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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