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| Status |
Public on Nov 16, 2020 |
| Title |
Next-generation sequencing analysis of circulating micro-RNA expression in response to parabolic flight as a spaceflight analogue |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
BACKGROUND: Understanding physiologic reactions to weightlessness is an indispensable requirement for safe human space missions. While adaptations of human organ systems in response to weightlessness have been described in former studies, their molecular background needs further elucidation. OBJECTIVE: The study aims to analyse changes in the expression of circulating miRNAs in serum in response to gravitational changes induced by parabolic flight as a spaceflight analogue. METHODS: Eight healthy volunteers (age: 25.4 years, male: 4, female: 4) were included. Each subject underwent 31 short-term phases of weightlessness and hypergravity induced by parabolic flight. At different time points (baseline, 1 hour after parabolic flight, and 24 hours parabolic flight), venous blood was withdrawn. Analysis of circulating miRNAs in serum was conducted by means of next generation sequencing. RESULTS: In total, 213 miRNAs were robustly detected by small RNA sequencing in all 24 samples. 4 miRNAs (mir-941, mir-24-3p, mir-486-5p, mir-223-3p) evidenced a significant change in expression after adjusting for multiple testing. mir-941 and mir-24-3p showed a significant decrease 24 hours after parabolic flight compared to 1 hour after parabolic flight. Contrary, mir-486-5p showed a significant increase 24 hours after parabolic flight compared to 1 hour after parabolic flight. Of note, mir-223-3p showed a significant decrease 24 hours after parabolic flight compared to baseline values and values at 1 hour after parabolic flight. A target network analysis identified genes of the p53 signaling pathway and the cell cycle highly enriched among the targets of the four microRNAs. CONCLUSIONS: Our findings suggest cellular adaption to gravitational changes by means of weightlessness and hypergravity already at the transcriptional level. Based on our results, we suggest a change in cell cycle regulation as potential explanation for adaptational changes observed in space missions.
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| Overall design |
24 samples were analyzed in total. These samples were obtained from 8 subjects at three different time points.
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| Contributor(s) |
Jirak P, Wernly B, Lichtenauer M, Franz M, Knost T, Abusamrah T, Malte K, Nana-Yaw B, Jung C |
| Citation(s) |
33298968 |
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https://doi.org/10.1038/s41526-020-00121-9
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| Submission date |
Mar 23, 2020 |
| Last update date |
Dec 15, 2020 |
| Contact name |
Matthias Hackl |
| E-mail(s) |
matthias.hackl@tamirna.com
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| Phone |
013913322
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| Organization name |
TAmiRNA GmbH
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| Street address |
Leberstrasse 20
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| City |
Vienna |
| State/province |
Austria |
| ZIP/Postal code |
1110 |
| Country |
Austria |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA614460 |
| SRA |
SRP253692 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE147380_P41-GEO-processed-data-file.xlsx |
74.6 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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