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| Status |
Public on Aug 10, 2020 |
| Title |
A role for alternative splicing in circadian control of insulin secretion and glucose homeostasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
We report the discovery of circadian clock-controlled alterantive pre-mRNA splicing in pancreatic beta cells and its role in insulin secretion. We performed RNA-sequencing in CRISPR-CAS9 edited Clock and Bmal1 knockout BetaTC6 cells and used differential mRNA expression and splicing analysis to identify and validate transcriptional and alternative splicing targets of the circadian clock regulating insulin secretion.
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| Overall design |
We have profiled the transcriptomes of wild type and Bmal1 knockout pancreatic beta cells by RNA-seq and performed ChIP-seq and eCLIP-seq to map the DNA and RNA binding positions of the RNA binding protein THRAP3 in these cells.
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| Contributor(s) |
Perelis M |
| Citation(s) |
32616519 |
| |
| Submission date |
Mar 12, 2020 |
| Last update date |
Aug 13, 2020 |
| Contact name |
Mark Perelis |
| E-mail(s) |
mperelis@ucsd.edu, perelis@gmail.com
|
| Phone |
8582461491
|
| Organization name |
University of California San Diego
|
| Street address |
Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Dr., Room 3805/Yeo Lab
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA612358 |
| SRA |
SRP252642 |
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