|
| Status |
Public on Sep 30, 2009 |
| Title |
GM-CSF-mediated granulopoiesis is regulated by the transcription factor STAT5A/B |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
GM-CSF controls the development of granulocytes but little is known about the contribution of the downstream mediating transcription factor STAT5A/B. To elucidate this pathway, we generated mice lacking the Stat5a and 5b genes in blood cells. Peripheral neutrophils were decreased and administration of 5-FU and GM-CSF failed to induce granulopoiesis in Stat5a/b-mutant mice. GMPs were isolated and cultured with GM-CSF. Both the number and size of STAT5A/B-null colonies were reduced and GM-CSF-induced survival of mature STAT5A/B-null neutrophils was impaired. Time-lapse cinematography and single cell tracking of GMPs revealed that STAT5A/B-null cells were characterized by a longer generation time and an increased cell death. Gene expression profiling experiments suggested that STAT5A/B directs GM-CSF signaling through the regulation of cell survival genes.
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| |
|
| Overall design |
Mice lacking or with the Stat5a and 5b genes in blood cells, which were treated w/o GMP
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| |
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| Contributor(s) |
Kimura A, Rieger M, Simone J, Chen W, Wickre M, O’Shea J, Schroeder T, Hennighausen L |
| Citation(s) |
19779039 |
| |
| Submission date |
Feb 02, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (12)
|
| GSM366044 |
03-25-08_Hennighausen_AK_Mouse430_2_GMPCTL- |
| GSM366045 |
03-25-08_Hennighausen_AK_Mouse430_2_GMPCTL+ |
| GSM366046 |
03-25-08_Hennighausen_AK_Mouse430_2_GMPKO+b |
| GSM366047 |
03-25-08_Hennighausen_AK_Mouse430_2_GMPKO- |
| GSM366048 |
08-05-08_Hennighausen_7AK_Mouse430_2_626_CG- |
| GSM366049 |
08-05-08_Hennighausen_8AK_Mouse430_2_626_CG+ |
| GSM366050 |
08-05-08_Hennighausen_3AK_Mouse430_2_626_KG- |
| GSM366051 |
08-05-08_Hennighausen_12AK_Mouse430_2_702_KG+ |
| GSM366052 |
09-26-08_LHennighausen1_AK_Mouse430_2_KOGMP+ |
| GSM366053 |
09-26-08_LHennighausen1_AK_Mouse430_2_KOGMP-C |
| GSM366054 |
09-26-08_LHennighausen1_AK_Mouse430_2_CTLGMP+ |
| GSM366055 |
09-26-08_LHennighausen1_AK_Mouse430_2_CTLGMP- |
|
| Relations |
| BioProject |
PRJNA112369 |
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