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| Status |
Public on Apr 11, 2020 |
| Title |
Integrated small RNA genomics of mosquito cells reveal dynamic evolutionary responses to viruses and transposons. |
| Organisms |
Aedes aegypti; Aedes albopictus; Anopheles gambiae; Culex quinquefasciatus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We present an integrated resource of profiling and analysis of small RNAs from mosquiot cells and tissues from 4 main species to determine how the viruses and transposons compare in the production of small regulatory RNAs.
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| |
|
| Overall design |
Various mosquito cell lines and tissues were cultured or obtained, dissected to separate gonads from soma, and then total RNA was extracted and the small RNAs were purified and converted to Illumina libraries for high throughput sequencing.
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| |
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| Contributor(s) |
Lau N, Ma Q, Srivastav S, Gamez S |
| Citation(s) |
33419731 |
| |
| Submission date |
Mar 06, 2020 |
| Last update date |
Mar 23, 2021 |
| Contact name |
NELSON LAU |
| E-mail(s) |
nclau@bu.edu
|
| Organization name |
Boston University School of Medicine
|
| Department |
Biochemistry
|
| Street address |
72 E Concord St K219, Dept of Biochemistry
|
| City |
BOSTON |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
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| Platforms (4)
|
| GPL22030 |
Illumina NextSeq 500 (Aedes aegypti) |
| GPL22031 |
Illumina NextSeq 500 (Aedes albopictus) |
| GPL22033 |
Illumina NextSeq 500 (Anopheles gambiae) |
| GPL22042 |
Illumina NextSeq 500 (Culex quinquefasciatus) |
|
| Samples (54)
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| Relations |
| BioProject |
PRJNA610833 |
| SRA |
SRP251875 |
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