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| Status |
Public on Nov 15, 2021 |
| Title |
Intestinal epithelial GR in response to DEX treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this project is to investigate the role of glucocorticoids and their receptor, glucocorticoid receptor (GR) , in intestinal stress response and tissue homeostasis.We generated a mouse model with specific deletion of GR in intestinal epithelium (GR iKO mice) and examined colonic transcriptomes of adrenalectomized (ADX) Flox control and GR iKO mice in response to dexamethasone (DEX) treatment by microarray analysis.
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| Overall design |
Total RNA were extracted from colon tissues of 4 pairs adrenalectomized (ADX) Flox control and GR iKO mice treated with or without 1 mg/Kg dexamethasone (DEX) for 6 hours. Gene expression profiles were analyzed using Agilent Whole Mouse Genome 4x44 multiplex format oligo arrays (014868) (Agilent Technologies, Santa Clara, CA), following the Agilent 1-color microarray-based gene expression analysis protocol.
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| Contributor(s) |
Li X, Tang S |
| Citation(s) |
34784298 |
| |
| Submission date |
Feb 28, 2020 |
| Last update date |
Nov 18, 2021 |
| Contact name |
Jian-Liang Li |
| Organization name |
NIEHS
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| Street address |
111 TW Alexander Dr
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA609257 |
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