|
| Status |
Public on Jul 22, 2020 |
| Title |
Single Cell Transcriptional Analysis of Donor and Recipient Immune Cell Chimerism in the Rejecting Kidney Transplant |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome variation profiling by high throughput sequencing
|
| Summary |
We analyzed the transcriptomes from 81139 cells from 5 kidney transplant biopsies. We sequenced the germline coding sequence from donor and recipient pairs corresponding to each biopsy as a reference to determine donor host chimerism in each kidney.
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| |
|
| Overall design |
10X Chromium and NGS used for single cell RNA-seq and Whole-exome sequencing respectfully.
|
| |
|
| Contributor(s) |
Malone AF |
| Citation(s) |
32669324 |
| |
| Submission date |
Feb 25, 2020 |
| Last update date |
Aug 22, 2023 |
| Contact name |
andrew malone |
| E-mail(s) |
78.andrew@gmail.com
|
| Phone |
3145045281
|
| Organization name |
Washington University
|
| Street address |
4525 laclede ave
|
| City |
st louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (17)
|
|
| Relations |
| BioProject |
PRJNA608747 |
| SRA |
SRP250734 |
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