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Series GSE145643 Query DataSets for GSE145643
Status Public on Feb 21, 2020
Title Functional characterization of thousands of type 2 diabetes-associated and chromatin-modulating variants under steady state and endoplasmic reticulum stress
Organisms Mus musculus; synthetic construct
Experiment type Other
Summary We tested >13,000 sequences containing each allele of 6,628 SNPs associated with altered in vivo chromatin accessibility in human islets and/or type 2 diabetes risk (T2D GWAS SNPs) for transcriptional activity in ß cell under steady state and endoplasmic reticulum (ER) stress conditions using the massively parallel reporter assay (MPRA).
Overall design MPRA in MIN6 treated with Thapsigargin or a solvent DMSO control
Contributor(s) Khetan S, Stitzel ML
Citation(s) 34475398
Submission date Feb 20, 2020
Last update date Sep 29, 2021
Contact name Shubham Khetan
Phone 8607943361
Organization name Brigham and Women's Hospital
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL19424 Illumina NextSeq 500 (synthetic construct)
Samples (9)
GSM4322895 Plasmid-Baseline
GSM4322896 RNA-Baseline
GSM4322897 Plasmid
BioProject PRJNA607788
SRA SRP250169

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145643_MIN6_MPRA_Baseline_FinalMatrix.out.txt.gz 449.4 Kb (ftp)(http) TXT
GSE145643_MIN6_MPRA_DMSO_TG_FinalMatrix_Run1.out.txt.gz 421.4 Kb (ftp)(http) TXT
GSE145643_MIN6_MPRA_DMSO_TG_FinalMatrix_Run2.out.txt.gz 418.9 Kb (ftp)(http) TXT
GSE145643_T2D_khetan_All_20180111.asi.probes.fa.gz 537.2 Kb (ftp)(http) FA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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