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| Status |
Public on Feb 21, 2020 |
| Title |
Functional characterization of thousands of type 2 diabetes-associated and chromatin-modulating variants under steady state and endoplasmic reticulum stress |
| Organisms |
Mus musculus; synthetic construct |
| Experiment type |
Other
|
| Summary |
We tested >13,000 sequences containing each allele of 6,628 SNPs associated with altered in vivo chromatin accessibility in human islets and/or type 2 diabetes risk (T2D GWAS SNPs) for transcriptional activity in ß cell under steady state and endoplasmic reticulum (ER) stress conditions using the massively parallel reporter assay (MPRA).
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| |
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| Overall design |
MPRA in MIN6 treated with Thapsigargin or a solvent DMSO control
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| |
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| Contributor(s) |
Khetan S, Stitzel ML |
| Citation(s) |
34475398 |
| |
| Submission date |
Feb 20, 2020 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Shubham Khetan |
| E-mail(s) |
skhetan@bwh.harvard.edu
|
| Phone |
8607943361
|
| Organization name |
Brigham and Women's Hospital
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL19424 |
Illumina NextSeq 500 (synthetic construct) |
|
| Samples (9)
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| Relations |
| BioProject |
PRJNA607788 |
| SRA |
SRP250169 |
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