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Series GSE14560 Query DataSets for GSE14560
Status Public on Mar 03, 2009
Title CLL: CD38+CD49d+ vs. CD38-CD49d-
Organism Homo sapiens
Experiment type Expression profiling by array
Summary CD38 and CD49d are associated negative prognosticators in chronic lymphocytic leukemia (CLL).
Despite evidence that both molecules are involved in interactions occurring between CLL and normal cells in the context of CLL-involved tissues, a functional link is still missing. Using gene expression profiles comparing CD38+CD49d+ vs. CD38-CD49d- CLL cells, we demonstrated
overexpression of the CCL3 and CCL4 chemokines in cells from the former group. These chemokines were also upregulated by CD38 signals in CLL; moreover, CCL3 was expressed by CLL cells from bone marrow biopsies (BMB) of CD38+CD49d+ but not CD38-CD49d- cases. High levels of CCR1 and, to a lesser extent, CCR5, the receptors for CCL3 and CCL4, were found in CLL-derived monocyte-macrophages. Consistently, CCL3 increased monocyte migration, and CD68+ macrophage infiltration was particularly high in BMB from CD38+CD49d+ CLL.
Conditioned media from CCL3-stimulated macrophages induced endothelial cells to express VCAM-1, the CD49d ligand, likely through TNF╬▒ over-production. These effects were apparent in BMB from CD38+CD49d+CLL, where lymphoid infiltrates were characterized by a prominent meshwork of VCAM-1+ stromal/endothelial cells. Lastly, CD49d engagement by VCAM-1-transfectants increased viability of CD38+CD49d+ CLL cells. Altogether, CD38 and CD49d can be thought of as a part of a consecutive chain of events ultimately leading to improved survival of CLL
Overall design Dual Color reference design with 26 different patients (15 cd38-cd49d- and 11 cd38+cd49d+)
Contributor(s) Zucchetto A, Benedetti D, Tripolo C, Bomben R, Dal Bo M, Marconi D, Gattei V, Gaidano G, Deagan M, Malavasi F, Lorenzon D, Rossi FM, Rossi D, Bulian P, Franco V, Del Poeta G, Tedesco F
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Submission date Jan 25, 2009
Last update date Feb 22, 2018
Contact name daniela marconi
Organization name CRO AVIANO
Street address Via franco Gallini 2
City Aviano
ZIP/Postal code 33081
Country Italy
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (26)
GSM361561 CD38-CD49d- 10139_2
GSM361996 CD38-CD49d- 10139_3
GSM362051 CD38-CD49d- 10163_2
BioProject PRJNA111739

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14560_RAW.tar 350.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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