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| Status |
Public on Aug 24, 2020 |
| Title |
Heparin anticoagulant for human bone marrow does not influence in vitro-performance of human mesenchymal stromal cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Background: Mesenchymal stromal cells (MSCs) are a promising cell source for tissue engineering and regenerative medicine. In our lab, we found that cell preparations from bone marrow of many donors had a limited capacity for in vitro-differentiation into the osteogenic and chondrogenic lineages although this is a capacity claimed to be inherent to this kind of cells. Therefore the current study was designed to test the hypothesis whether the amount of heparin used as anti-coagulant during bone marrow harvest has inhibitory influence on the in vitro-differentiation capacity of the isolated MSCs. Methods: Bone marrow was obtained from twelve donors with good physical condition during preparation of the femoral cavity for the implantation of the femoral stem for a total hip arthroplasty in the absence or presence of heparin. Isolated MSCs were characterized by plastic adhesion, morphology, population doubling times, expression of cell surface antigens and in vitro-differentiation into the adipogenic, osteogenic and chondrogenic lineages. In addition, transcriptome analyses were performed. Results: Bone marrow prepared in the absence of heparin showed a comparable processability as bone marrow in the presence of heparin. Notably, no coagulation was observed. The number of mononuclear cells retrieved from the density gradient was independent of heparin addition. Moreover, none of the phenotypic or functional parameters assessed correlated with the addition of heparin. Transcriptome and quantitative realtime PCR analyses demonstrated that some selected genes were up- or downregulated in the cells isolated with the addition of heparin. However, this statement held true for only some but not all of the donors investigated in the present study. Conclusions: No correlation with heparin addition was observed with respect to the parameters assessed in the present study. This implies the absence of consequences both for the results of in vitro-investigations and also during in vivo-application, thereby ruling out heparin as a potential source of disparate results that are described in the literature.
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| Overall design |
Mesenchymal stromal cells from bone marrow of three donors were treated with heparin or were left untreated. Accordingly, six samples were processed and finally analyzed by use of single color microarray technology.
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| Contributor(s) |
Roger Y, Dittrich-Breiholz O, Hoffmann A, Burmeister L, Hamm A, Elger K, Flörkemeier T |
| Citation(s) |
32610653 |
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| Submission date |
Feb 18, 2020 |
| Last update date |
Aug 24, 2020 |
| Contact name |
Oliver Dittrich-Breiholz |
| E-mail(s) |
dittrich.oliver@mh-hannover.de
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| Organization name |
Medical School Hannover
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| Department |
Research Core Unit Genomics
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| Street address |
Carl-Neuberg-Str. 1
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| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL26355 |
Agilent-084555 026652QM_RCUG_HomoSapiens (Probe Name Version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA607215 |
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