|
| Status |
Public on Feb 14, 2020 |
| Title |
RNA sequencing LSK cells after Cxxc5 knockdown |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To study the effect of knockdown of Cxxc5 on stem/progenitor cells, we used retrovirus to knockdown Cxxc5 expression in the LSK cells. We used this as a surrogate to study the function of Cxxc5 in mouse/stem progenitor cells.
|
| |
|
| Overall design |
Stem/progenitor cells enriched bone marrow cells from BALB/c mice were transduced with retrovirus to knockdown Cxxc5. Retrovirus that does not target any mouse genes was used as a control. Infected LSK cells (GFP positive LSK) were flow-sorted. Sorted infected LSK cells from multiple animals were pooled together for RNA isolation.
|
| |
|
| Contributor(s) |
Joshi HR, Kumanovics A, Stubben C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 13, 2020 |
| Last update date |
Feb 15, 2020 |
| Contact name |
Chris Stubben |
| Organization name |
Huntsman Cancer Institute
|
| Department |
University of Utah
|
| Street address |
2000 Cir of Hope Dr
|
| City |
Salt Lake City |
| State/province |
Utah |
| ZIP/Postal code |
84103 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA606512 |
| SRA |
SRP249287 |
Less...
More...