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| Status |
Public on Feb 14, 2020 |
| Title |
High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here, the effects of high glucose levels on influenza severity were investigated using an in vitro model of the pulmonary epithelial-endothelial barrier. We show that compared to low glucose levels, high glucose conditions prior to influenza A virus infection increased virus-induced barrier damage. Increased barrier damage was not associated with increased cell death nor increased virus replication, but rather an increased pro-inflammatory response in endothelial cells and the subsequent damage of the epithelial junctional complex.
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| Overall design |
Human epithelial cells and human pulmonary microvascular endothelial cells were grown in a transwell system. Endothelial cells were exposed to either normoglycemic (7mM glucose) or hyperglycaemic conditions (12mM glucose). Cells were then infected with Influenza A Virus (or mock infection protocol for control) before performing RNA-Seq analysis.
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| Contributor(s) |
Short KR, Upton KR |
| Citation(s) |
32697191 |
| |
| Submission date |
Feb 13, 2020 |
| Last update date |
Aug 03, 2020 |
| Contact name |
Kyle R Upton |
| E-mail(s) |
kyle.upton.is@gmail.com
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| Organization name |
University of Queensland
|
| Department |
SCMB
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| Lab |
Upton
|
| Street address |
Cooper Road
|
| City |
Brisbane |
| State/province |
Qld |
| ZIP/Postal code |
4067 |
| Country |
Australia |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA606471 |
| SRA |
SRP248940 |
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