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| Status |
Public on Mar 22, 2020 |
| Title |
Transcriptional responses to TNF following HFol treatment of wild type and GCN2 depleted synoviocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the effects of Hfol on TNF induction of inflammatory genes in wild type cells versus cells depleted of GCN2
RNA-seq analysis of wild type or GCN2-depleted established fibroblast like synoviocytes (K4 cells) treated with TNFa in the presence or absence of Halofuginone (HF).
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| Overall design |
To test the requirement for the protein kinase GCN2 in the effects of the aminoacyl tRNA synthetase (AARS) inhibitor HF, wild type K4 cells or cells depleted of GCN2 by stable infection with a lentiviral shRNA directed against GCN2 cells in culture were treated, using duplicate samples for each condition, for 16 hours with HF and 8 hours with TNFa.
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| Contributor(s) |
Keller T, Whitman M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 12, 2020 |
| Last update date |
Mar 22, 2020 |
| Contact name |
John N Hutchinson |
| Organization name |
Harvard Chan School of Public Health
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| Street address |
677 Huntington Avenue
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE145205 |
Transcriptional responses of fibroblast like synoviocytes to TNFa in the presence or absence of Halofuginone (HF) |
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| Relations |
| BioProject |
PRJNA606297 |
| SRA |
SRP248465 |
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