|
| Status |
Public on Feb 09, 2020 |
| Title |
Single cell RNA-seq of mouse dorsal raphe Pet1 neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here we use high-throughput and DR subdomain-targeted single-cell transcriptomics and intersectional genetic tools to map molecular and anatomical diversity of DR-Pet1 neurons. We describe up to fourteen neuron subtypes, many showing biased cell body distributions across the DR.
|
| |
|
| Overall design |
Single cell RNA-seq libraries prepared from genetically labeled Pet1 neurons in two different ways: (1) automated fluorescence-based cell sorting (using the On-Chip Sort) followed by 10X Genomics Chromium Single Cell 3’ v3 protocol (samples 71-72), or (2) DR subdomain-targeted manual cell sorting, followed by SMART-Seq v4 Ultra Low Input cDNA amplification and Nextera XT library preparation (samples 1-70).
|
| |
|
| Contributor(s) |
Okaty BW, Sturrock N |
| Citation(s) |
32568072 |
| |
| Submission date |
Feb 08, 2020 |
| Last update date |
Jun 29, 2020 |
| Contact name |
Benjamin Okaty |
| E-mail(s) |
bokaty@genetics.med.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (72)
|
|
| Relations |
| BioProject |
PRJNA605552 |
| SRA |
SRP247768 |
Less...
More...