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| Status |
Public on Nov 18, 2020 |
| Title |
High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human embryonic stem cell gastruloid cultures |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used circular micropattern cultures to differentiate human embryonic stem cells (H1 hESCs) into microcolonies termed ‘gastruloids’, comprising germ layer and extraembryonic cells. Differentiated gastruloids were dissociated and reseeded onto micropatterns in single cells solution to study cell sorting behaviors. We applied single-cell RNA sequencing to examine transcriptomes of gastruloids and reseeded cells.
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| Overall design |
Single-cell RNA sequencing was performed on gastruloid cells differentiated from H1 hESCs after 44 hours of BMP4 treatment in micropatterns, and dissociated gastruloid cells reseeded onto micropatterns for 48 hours.
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| Contributor(s) |
Morris SA |
| Citation(s) |
33206048, 33891870 |
| |
| Submission date |
Feb 06, 2020 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Samantha Morris |
| E-mail(s) |
s.morris@wustl.edu
|
| Phone |
3147478618
|
| Organization name |
Washington University in St Louis
|
| Street address |
Department of Developmental Biology, Washington University School of
|
| City |
St Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA605221 |
| SRA |
SRP247607 |
