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Series GSE144841 Query DataSets for GSE144841
Status Public on Oct 23, 2020
Title 4EHP and GIGYF1/2 induce translation-coupled messenger RNA decay
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Although canonical mRNA degradation is generally recognized to be translation dependent, our understanding of the molecular events that coordinate ribosome movement with the decay machinery is still limited. Here, we show that the 4EHP–GIGYF1/2 complex triggers co-translational mRNA decay as a result of altered ribosome activity during elongation. Human cells lacking 4EHP and GIGYF1 and 2 proteins accumulate transcripts known to be degraded in a translation dependent manner or with prominent ribosome pausing. These include mRNAs encoding secretory and membrane-bound proteins or specific tubulin isotypes, among others. 4EHP–GIGYF1/2 fails to reduce target mRNA levels in the absence of ribosome stalling or upon disruption of its interaction with the cap structure, DDX6 and a GYF domain-associated protein. Our studies reveal how a repressor complex linked to neurological disorders minimizes the protein output of a subset of mRNAs.
Overall design Three cell types with two biological replicates analysed using RNA-Seq and Ribo-Seq
Contributor(s) Weber R, Igreja C
Citation(s) 33053355
Submission date Feb 06, 2020
Last update date Jan 22, 2021
Contact name Ramona Weber
Organization name University of Zurich
Department Institute for Regenerative Medicine
Street address Wagistrasse 12
City Schlieren
ZIP/Postal code 8952
Country Switzerland
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (12)
GSM4299125 Wild-type replicate 1 for RNA-seq
GSM4299126 Wild-type replicate 2 for RNA-seq
GSM4299127 4EHP-null replicate 1 for RNA-seq
BioProject PRJNA605114
SRA SRP247494

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE144841_DE-RNA_4EHP_vs_wt.txt.gz 398.3 Kb (ftp)(http) TXT
GSE144841_DE-RNA_GIGYF_vs_wt.txt.gz 396.3 Kb (ftp)(http) TXT
GSE144841_DE-Ribo_4EHP_vs_wt.txt.gz 364.2 Kb (ftp)(http) TXT
GSE144841_DE-Ribo_GIGYF_vs_wt.txt.gz 361.8 Kb (ftp)(http) TXT
GSE144841_TE_4EHP_vs_wt.txt.gz 404.2 Kb (ftp)(http) TXT
GSE144841_TE_GIGYF_vs_wt.txt.gz 385.8 Kb (ftp)(http) TXT
GSE144841_readcounts_RNA.txt.gz 343.6 Kb (ftp)(http) TXT
GSE144841_readcounts_Ribo.txt.gz 308.0 Kb (ftp)(http) TXT
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Processed data are available on Series record

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