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| Status |
Public on Feb 06, 2020 |
| Title |
Global CpG methylation analysis of primary endothelial cells before and after TNFa stimulation |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by array
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| Summary |
Endothelial cells are a primary site of leukocyte recruitment during inflammation. An increase in tumor necrosis factor-alpha (TNFa) levels as a result of infection or some autoimmune diseases can trigger this process. Several autoimmune diseases are now treated with TNFa inhibitors. However, genomic alterations that occur as a result of TNF-mediated inflammation are not well understood. To investigate molecular targets and networks resulting from increased TNFa, we measured DNA methylation and gene expression in 40 human umbilical vein endothelial cell primary cell lines before and 24 hours after stimulation with TNFa via microarray.
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| Overall design |
The final dataset used for analysis consisted of 37 sample pairs and 818,391 CpG sites.
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| Contributor(s) |
Rhead B, Shao X, Quach H, Ghai P, Barcellos LF, Bowcock AM |
| Citation(s) |
32231389 |
| |
| Submission date |
Feb 05, 2020 |
| Last update date |
May 07, 2020 |
| Contact name |
Anne M Bowcock |
| E-mail(s) |
anne.bowcock@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Street address |
One Gustave L. Levy Place, Box 1130
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL23976 |
Illumina Infinium HumanMethylation850 BeadChip |
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| Samples (74)
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| This SubSeries is part of SuperSeries: |
| GSE144810 |
Analysis of primary endothelial cells before and after TNFa stimulation |
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| Relations |
| BioProject |
PRJNA604987 |
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