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| Status |
Public on Mar 01, 2020 |
| Title |
Drosophila Estrogen-Related Receptor directs a transcriptional switch that supports adult glycolysis and lipogenesis [ChIP-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Metabolism and development must be closely coupled to meet the changing physiological needs of each stage in the life cycle. The molecular mechanisms that link these pathways, however, remain poorly understood. Here we show that the Drosophila Estrogen-Related Receptor (dERR) directs a transcriptional switch in mid-pupae that promotes glucose oxidation and lipogenesis in young adults. dERR mutant adults are viable but display reduced locomotor activity, susceptibility to starvation, elevated glucose and an almost complete lack of stored triglycerides. Molecular profiling by RNA-seq, ChIP-seq, and metabolomics revealed that glycolytic and pentose phosphate pathway genes are induced by dERR, and their reduced expression in mutants is accompanied by elevated glycolytic intermediates, reduced TCA cycle intermediates, and reduced levels of long chain fatty acids. Unexpectedly, we find that the central pathways of energy metabolism, including glycolysis, the tricarboxylic acid cycle, and electron transport chain, are coordinately induced at the transcriptional level in mid-pupae and maintained into adulthood, and this response is partially dependent on dERR, leading to the metabolic defects observed in mutants. Our data support the model that dERR contributes to a transcriptional switch during pupal development that establishes the metabolic state of the adult fly.
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| Overall design |
ChIP-seq comparison of enriched loci between ERR-FLAG tagged animals and controls.
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| Contributor(s) |
Thummel CS, Beebe K |
| Citation missing |
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| |
| Submission date |
Feb 04, 2020 |
| Last update date |
Mar 01, 2020 |
| Contact name |
Katherine Beebe |
| E-mail(s) |
kbeebe@genetics.utah.edu
|
| Organization name |
University of Utah
|
| Department |
Molecular Medicine
|
| Lab |
Dr. Aylin Rodan
|
| Street address |
15 North 2030 East
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE144766 |
Drosophila Estrogen-Related Receptor directs a transcriptional switch that supports adult glycolysis and lipogenesis |
|
| Relations |
| BioProject |
PRJNA604806 |
| SRA |
SRP247186 |
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