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Series GSE144762 Query DataSets for GSE144762
Status Public on Mar 01, 2020
Title Drosophila Estrogen-Related Receptor directs a transcriptional switch that supports adult glycolysis and lipogenesis [RNA-Seq d6]
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Metabolism and development must be closely coupled to meet the changing physiological needs of each stage in the life cycle. The molecular mechanisms that link these pathways, however, remain poorly understood. Here we show that the Drosophila Estrogen-Related Receptor (dERR) directs a transcriptional switch in mid-pupae that promotes glucose oxidation and lipogenesis in young adults. dERR mutant adults are viable but display reduced locomotor activity, susceptibility to starvation, elevated glucose and an almost complete lack of stored triglycerides. Molecular profiling by RNA-seq, ChIP-seq, and metabolomics revealed that glycolytic and pentose phosphate pathway genes are induced by dERR, and their reduced expression in mutants is accompanied by elevated glycolytic intermediates, reduced TCA cycle intermediates, and reduced levels of long chain fatty acids. Unexpectedly, we find that the central pathways of energy metabolism, including glycolysis, the tricarboxylic acid cycle, and electron transport chain, are coordinately induced at the transcriptional level in mid-pupae and maintained into adulthood, and this response is partially dependent on dERR, leading to the metabolic defects observed in mutants. Our data support the model that dERR contributes to a transcriptional switch during pupal development that establishes the metabolic state of the adult fly.
 
Overall design Comparison of mRNA levels in 6 day old control and dERR mutant adults. n=3 replicates per treatment
 
Contributor(s) Thummel CS, Beebe K
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Submission date Feb 04, 2020
Last update date Mar 01, 2020
Contact name Katherine Beebe
E-mail(s) kbeebe@genetics.utah.edu
Organization name University of Utah
Department Molecular Medicine
Lab Dr. Aylin Rodan
Street address 15 North 2030 East
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (6)
GSM4295290 13499X10
GSM4295291 13499X11
GSM4295292 13499X12
This SubSeries is part of SuperSeries:
GSE144766 Drosophila Estrogen-Related Receptor directs a transcriptional switch that supports adult glycolysis and lipogenesis
Relations
BioProject PRJNA604807
SRA SRP247185

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE144762_13499R_RNAseq_d6.xlsx 2.9 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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