|
| Status |
Public on Jan 24, 2022 |
| Title |
mTEClo/int RNAseq profiling in three mouse models of impaired mTEC/Tcell crosstalk |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
mTEC/Tcell crosstalk drives transcriptional and epigenetic programs conditioning immunological tolerance
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| |
|
| Overall design |
Total RNA was isolated with miRNeasy Micro Kit (Qiagen) from mTEClo/int cells (EpCAM+ UEA-1+ Ly51lo CD80lo/int) from individual mice of three different mouse models in which the mTEC/Tcell crosstalk is impaired: CIITA-KO [CIIta(III+IV)-/-] vs WT ; K14xCIITAKO vs WT and RipmOVAxOTII vs OTII. The extracted RNA was used to generate transcriptome libraries using the SMART-Seq-v4-Ultra Low Input RNA Kit (Clontech) combined to the Nextera library preparation kit (Illumina) following the manufacturer's protocols. Single-end (75 bp) sequencing was performed using the Illumina NextSeq 500 machine.
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| |
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| Contributor(s) |
Irla M, Giraud M |
| Citation(s) |
35188458 |
| |
| Submission date |
Feb 02, 2020 |
| Last update date |
Mar 02, 2022 |
| Contact name |
Matthieu Giraud |
| E-mail(s) |
matthieu.giraud@inserm.fr
|
| Organization name |
INSERM _ Université de Nantes
|
| Department |
CRTI
|
| Street address |
30 bd Jean Monnet
|
| City |
Nantes |
| ZIP/Postal code |
44000 |
| Country |
France |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (10)
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|
| Relations |
| BioProject |
PRJNA604453 |
| SRA |
SRP246692 |
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