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| Status |
Public on Dec 10, 2021 |
| Title |
HIF-1a tunes T follicular helper cell differentiation during acute viral infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
T follicular helper (Tfh) cells provide signals to initiate and maintain the germinal center (GC) reaction and are crucial for the generation of robust antibody responses. We found that hypoxia inducible factor-1 (HIF-1a) is an inhiibitor of Tfh generation in a mouse model of acute viral infection, and that HIF-1a mediates its effects by suppressing mTORC1 activity.
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| |
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| Overall design |
Bulk RNA-seq of WT or HIF-1a knockout LCMV-specific CD4 T cells, sorted into Th1, pre-Tfh, and GC Tfh populations.
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| |
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| Contributor(s) |
Huang B, Jeffrey B, Schwartzberg PL |
| Citation(s) |
35145086 |
| |
| Submission date |
Jan 29, 2020 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Brendan McNie Jeffrey |
| E-mail(s) |
brendan.jeffrey@nih.gov
|
| Phone |
406-375-9702
|
| Organization name |
NIH
|
| Department |
BCBB
|
| Street address |
903 South 4th Street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (24)
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| Relations |
| BioProject |
PRJNA603744 |
| SRA |
SRP245855 |
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