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Series GSE144455 Query DataSets for GSE144455
Status Public on Apr 16, 2021
Title Effect of single MgSO4 injection on brain transcripts in naive and ishemic 5 day-old mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary MgSO4 is given to prevent preterms neurological deficits. We have investigated proper effects and hypoxia-ischemia interfering effects of MgSO4 on transcription in neonate mice at day 5 (a brain development stage mimicking preterm maturity), at neuroprotection dose (600 mg/kg).
Pools of RNA from 6 forebrains (3 males and 3 females) were used for total RNA extraction at 5 times post single MgSO4 injection (1.5H, 3H, 6H, 12H, 24H). Separately, MgSO4 effects were assessed post hypoxia-ischemia (3H and 12H). Co-hybridization of MgSO4-treated mRNA (labelled Cy5) and control tissues mRNA (labelled Cy3) were performed on Agilent (design 026655) micro-arrays in 4-7 replicates. Co-hybridization of saline injected (Labelled Cy3) or MgSO4 injected (Labelled Cy5) hypoxia-ischemia (according to Rice Vannucci (RV) procedure) exposed mice brains were performed on Aglient (design 026655) microarrays in triplicates.
 
Overall design Two color experiments : MgSO4 treatment (Cy5)/matched time post Phosphate buffer saline injections (Cy3) and Hypoxia-ischmia brains from saline treated (Cy3) and MgSO4 treated (Cy5) mice.
 
Contributor(s) Le Dieu-Lugon B, Derambure C, Dupré N, Stéphane M, Leroux P
Citation(s) 33923910
Submission date Jan 29, 2020
Last update date May 06, 2021
Contact name Celine Derambure
E-mail(s) celine.derambure1@univ-rouen.fr
Organization name INSERM U905
Street address 22 Bvd Gambetta
City Rouen cedex
ZIP/Postal code 76183
Country France
 
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (35)
GSM4288501 Effect of MgSO4 + 1h30 [Mg 1h30 a]
GSM4288502 Effect of MgSO4 + 1h30 [Mg 1h30 b]
GSM4288503 Effect of MgSO4 + 1h30 [Mg 1h30 c]
Relations
BioProject PRJNA603700

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE144455_RAW.tar 118.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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