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| Status |
Public on Mar 11, 2020 |
| Title |
Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Classical dendritic cells (cDCs) are rare sentinel cells specialized in the regulation of adaptive immunity. Modelling cDC development is both crucial to study cDCs and harness their potential in immunotherapy. Here we present a novel in vitro cDC differentiation protocol using cord blood CD34+ hematopoietic stem and progenitor cells (HSPCs) co-cultured with bone marrow-derived murine mesenchymal cell line (MS5) engineered to co-express human FLT3L, SCF and CXCL12 (MS5_FS12).
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| Overall design |
Blood cDC1 and cDC2 were isolated from healthy donor buffy coats. In vitro generated cDC1, cDC2 CD206- and cDC2 CD206+ cells were collected at day 14. All cell types were analysed in triplicates with up to 100 cells per sample.
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| Contributor(s) |
Pierre G, Giorgio A, Kristine V |
| Citation(s) |
32345968 |
| |
| Submission date |
Jan 29, 2020 |
| Last update date |
May 12, 2020 |
| Contact name |
Kristine Vaivode |
| E-mail(s) |
kristine.vaivode@kcl.ac.uk
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| Phone |
02078486907
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| Organization name |
King's College London
|
| Department |
Centre for Inflammation Biology and Cancer Immunology
|
| Lab |
Dr Pierre Guermonprez lab
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| Street address |
CIBCI, First Floor, New Hunt's House, Great Maze Pond
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| City |
London |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA603654 |
| SRA |
SRP245802 |
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