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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 28, 2020 |
| Title |
Microarray analysis of Med23 mutation in E9.5 mouse embryos relative to control littermates |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Development of the vertebrate head is a complex and dynamic process, which requires integration of all three germ layers and their derivatives. Of special importance are ectoderm-derived cells that form the cranial placodes, which then differentiate into the cranial ganglia and sensory organs. Critical to a fully functioning head, defects in cranial placode and sensory organ development can result in congenital craniofacial anomalies. In a forward genetic screen aimed at identifying novel regulators of craniofacial development, we discovered an embryonically lethal mouse mutant, snouty, which exhibits malformation of the facial prominences, cranial nerves and vasculature. The snouty mutation was mapped to a single nucleotide change in a ubiquitously expressed gene, Med23, which encodes a subunit of the global transcription co-factor complex, Mediator. This experiment examines transcriptional differences between Med23 mutant mice and their wt littermates at embryonic day 9.5.
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| Overall design |
Two wt and three Med23 mutant embryos are compared across 6 microarrays using a dye swap strategy. The third mutant is compared to a mixture of the 1st and 2nd wt replicates.
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| Contributor(s) |
Dash S, Seidel CW, Bhatt S, Sandell L, Ahn Y, Krumlauf R, Trainor P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 27, 2020 |
| Last update date |
Jan 30, 2020 |
| Contact name |
Chris W Seidel |
| E-mail(s) |
seidel@phageT4.org
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| Phone |
816 926 9054
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| Organization name |
Stowers Institute
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| Department |
Genomics
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| Lab |
Seidel
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| Street address |
1000 E 50th St
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| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
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| Platforms (1) |
| GPL28083 |
SIMR Mouse 40176 MEBO2_10-9-2007 |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA603374 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE144327_RAW.tar |
37.9 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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