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| Status |
Public on Apr 17, 2009 |
| Title |
A C. elegans LSD1 Demethylase Contributes to Germline Immortality by Reprogramming Epigenetic Memory |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray-based expression profiling of mixed stage populations taken from generation 1,13 and 26 spr-5 mutant animals as well as wild-type animals reveals a large class of spermatogenesis-expressed genes whose expression coordinately increased from generations 1 to 13 and then decreased from generations 13 to 26 in spr-5(by101) mutants. These results suggest that a failure to reset spermatogenesis acquired H3K4me2 may result in the progressive sterility that is observed in spr-5 mutants.
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| Overall design |
8ug of total RNA was hybridized pair-wise, along with a dye flip, from N2 (wild-type) f1 and spr-5(by101) mutant f1, f13 and f26, for a total of 12 arrays. RNA was labelled with the Genisphere Array350 system.
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| Contributor(s) |
Katz DJ, Edwards TM, Reinke V, Kelly WG |
| Citation(s) |
19379696 |
| |
| Submission date |
Jan 14, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
David John Katz |
| E-mail(s) |
djkatz@emory.edu
|
| Phone |
4047274580
|
| Fax |
4047272880
|
| Organization name |
Emory University
|
| Department |
Biology
|
| Lab |
Kelly
|
| Street address |
Rollins Bldg 1510 Clifton Rd
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA111763 |
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