|
| Status |
Public on Jan 24, 2020 |
| Title |
Remodeling of Zn2+ Homeostasis Upon Differentiation of Mammary Epithelial Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using mouse mammary epithelial cells as a model system, we investigated the remodeling of zinc homeostasis during differentiation induced by treatment with the lactogenic hormones cortisol and prolactin. RNA-Seq at different stages of differentiation revealed changes in global gene expression, including genes encoding zinc-dependent proteins and regulators of zinc homeostasis. Increases in mRNA levels of three zinc homeostasis genes,.
|
| |
|
| Overall design |
3 replicates of each of 3 sample groups (R day1, D day1, D day6). R day1 was used as the reference condition for all comparisons.
|
| |
|
| Contributor(s) |
Palmer AE, Han Y |
| Citation(s) |
31950952 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| DP1 GM114863 |
Regulation of Cell Signaling by Transition Metal Dynamics |
UNIVERSITY OF COLORADO AT BOULDER |
Amy E Palmer |
| T32 GM008759 |
Predoctoral Training in Signaling and Cellular Regulation |
UNIVERSITY OF COLORADO AT BOULDER |
Natalie G Ahn |
|
| |
| Submission date |
Jan 23, 2020 |
| Last update date |
Jan 25, 2020 |
| Contact name |
Yu Han |
| E-mail(s) |
yu.han@cuanschutz.edu
|
| Organization name |
University of Colorado
|
| Street address |
12700 E. 19th Ave Mail Stop-B139
|
| City |
Aurora |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA602910 |
| SRA |
SRP244438 |
Less...
More...