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| Status |
Public on Jan 24, 2020 |
| Title |
A Balance Between Intermediate Filaments and Microtubules Maintains Nuclear Architecture in the Cardiomyocyte |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In this manuscript we reported a unique role for desmin in the maintence of nuclear morphology. We performed this LaminB chip-seq to determine if there was a change in LAD organization with desmin KD.
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| Overall design |
LaminB chip-seq was performed from n=3 biological replicates of scrambled (control) or shDesmin treated cells. Each ChIP was paired with an input, and after sequencing, all three replicates were unionized. After input normalization, BigWig files were generated from the control and shDesmin conditions. A bed file of LADs in the control sample is included; no LADs were defined in the shDesmin condition. Some libraries were re-run as noted below.
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| Contributor(s) |
Heffler J, Shah PP, Robison P, Phyo S, Veliz K, Uchida K, Bogush A, Rhoades J, Jain R, Prosser BL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 23, 2020 |
| Last update date |
Jan 25, 2020 |
| Contact name |
Julie Heffler |
| E-mail(s) |
julieheffler@gmail.com
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| Organization name |
University of Pennsylvania
|
| Department |
Physiology
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| Lab |
Prosser
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| Street address |
415 Curie Blvd
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA602909 |
| SRA |
SRP244434 |
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