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| Status |
Public on Jan 24, 2020 |
| Title |
In Situ on DMSO and EPZ6438 treated CD34+ HSPCs |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
The overall goal is to understand how does the DNA methylation Canyon interactions in CD34+ HSPCs can be impacted by the inhibition of EZH2
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| |
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| Overall design |
We has thus treat the CD34+ HSPCs in the ex vivo culture condition with DMSO and EZH2 inhibitor, we then utilzed the in situ HiC to detect the interactions of chromosomal loops, DNA methylation Canyons.We also use H3K27me3 ChIP-seq to detect the H3K27me3 in the EZH2 inhibitor treatment setting and use RNA-seq to test if the DNA methylation Canyon associated genes will be altered in the gene expresion
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| |
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| Contributor(s) |
Zhang X, Gore H |
| Citation(s) |
32386543 |
| |
| Submission date |
Jan 23, 2020 |
| Last update date |
Jul 16, 2020 |
| Contact name |
Xiaotian Zhang |
| E-mail(s) |
zxtzhangqian@gmail.com
|
| Organization name |
The University of Texas Health Science Center at Houston
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Zhang Lab
|
| Street address |
6431 Fannin St
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA602860 |
| SRA |
SRP244334 |
