Expression profiling by high throughput sequencing Methylation profiling by high throughput sequencing
Summary
m6A-seq was performed on 4 brain regions (cortex, cerebellum, hypothalmus and hippocampus) of 2 week, 4 week, 6 week, 26 week and 52 week old BL6 mice. m6A profiling was also performed on human adolescent and old brain tissue (region BA9). m6A-seq was also performed on WT and 5xFAD mice (Alzheimer).
Overall design
RNA was extracted from the brain tissues in biologcial duplicates. m6A-seq was completed on these samples using a m6A-antibody coupled with NGS. RNA-seq of the same samples are also included to serve as a background for m6A peak calling.
Less...
More...