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| Status |
Public on Apr 15, 2020 |
| Title |
Microarray of E. coli-infected bone marrow-derived dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dendritic cells (DCs) are critical mediators of host defense against bacteria. The goal of this microarray study was to understand the global transcriptional response of bone marrow-derived dendritic cells (BMDCs) upon exposure to live bacteria, to better understand how DCs orchestrate a host-protective immune response. We found that BMDCs upregulate a number of critical immune-related genes upon exposure to live E. coli. Most notably, the gene encoding hepcidin, a critical regulator of mammalian iron homeostasis, was significantly upregulated in BMDCs upon exposure to live bacteria.
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| Overall design |
Microarray of E. coli-infected bone marrow-derived dendritic cells
Bone marrow-derived dendritic cells were infected with E. coli and cells were analyzed on day 4 post-infection
3 replicates were performed
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| Contributor(s) |
Sonnenberg GF, Fung TC, Bessman NJ |
| Citation(s) |
32273468 |
| |
| Submission date |
Jan 17, 2020 |
| Last update date |
Apr 17, 2020 |
| Contact name |
Gregory Putzel |
| Organization name |
NYU Langone Health
|
| Department |
Microbiology
|
| Lab |
Pironti
|
| Street address |
430 East 29th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA601923 |
