|
| Status |
Public on Jan 15, 2020 |
| Title |
C/EBPα is crucial determinant of epithelial maintenance by preventing epithelial-to-mesenchymal transition |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-sequencing of human mammary epithelial cells (HMLEs) that were lentivirally transduced with vector that constitutively expresses C/EBPα and its respective empty vector control. Both lines were analyzed with and without TGF-β treatment.
|
| |
|
| Overall design |
RNA-sequencing of human mammary epithelial cells (HMLEs) engineered to express either pLZRS EV or pLZRS-C/EBPα with and without TGF-β (2.5ng/ml) for 24 hours.
Through RNA-sequencing we analysed the differentially TGF-β regulated genes that were also regulated by C/EBPα
|
| |
|
| Contributor(s) |
Roukens MG, Lourenço AR, Coffer PJ |
| Citation(s) |
32034145 |
| |
| Submission date |
Jan 14, 2020 |
| Last update date |
Apr 15, 2020 |
| Contact name |
Ana Lourenço |
| E-mail(s) |
a.rita.lou@gmail.com, m.g.roukens-2@umcutrecht.nl
|
| Organization name |
UMC Utrecht
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA601109 |
| SRA |
SRP241893 |
Less...
More...