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| Status |
Public on Oct 09, 2020 |
| Title |
Loss of the RNA trimethylguanosine cap is compatible with nuclear accumulation of spliceosomal snRNAs but not pre-mRNA splicing or snRNA processing during animal development |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The 2,2,7-trimethylguanosine (TMG) cap is synthesized by the conserved Tgs1 enzyme, which is abundantly present on snRNAs essential for pre-mRNA splicing. In our study, we characterize new mutations in the Drosophila tgs1 gene and studied their effects on development and on splicing. Utilizing a hypomorphic tgs1 mutation, we uncovered a prominent role of Tgs1 in pre-mRNA splicing, particularly of introns with smaller sizes and weaker splice sites. Remarkably, stronger tgs1 mutations generated by CRISPR-Cas9 cause lethality without disrupting splicing, likely due to the preponderance of a maternal contribution and stable TMG capped snRNAs maintaining a near normal level of TMG modified snRNAs, which further proved that the TMG modification is important in splicing.
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| Overall design |
8 samples in total; 4 kinds of RNA samples from wildtype and tgs1 mutant animals were prepared and sequenced with two replicates.
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| Contributor(s) |
Cheng L, Zhang Y, Zhang Y, Chen T, Xu Y, Rong YS |
| Citation(s) |
33085660 |
| |
| Submission date |
Jan 12, 2020 |
| Last update date |
Jan 08, 2021 |
| Contact name |
Lin Cheng |
| E-mail(s) |
chengl28@mail2.sysu.edu.cn
|
| Organization name |
Sun Yat-sen University
|
| Street address |
No.132, East Outer Ring Road, Guangzhou University City
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510006 |
| Country |
China |
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| Platforms (1) |
| GPL23702 |
HiSeq X Ten (Drosophila melanogaster) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA600752 |
| SRA |
SRP241647 |
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