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Status |
Public on Oct 12, 2021 |
Title |
GABAergic neuronal IL-4R mediates T cell effect on memory [Il4raFlox] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The immune and nervous systems are highly interactive, however, little is known about how immune cells and their derived molecules impact brain function. Here we show that T cells play a critical role in retrieval of contextual fear conditioning (CFC) memory. Single-nuclei sequencing (snRNAseq) of engram neurons and non-engram neurons from the dentate gyrus one day after CFC learning revealed new evidence about differential expression of immune-related and synaptic plasticity-related genes, in SCID vs. wild-type mice. These findings present a comprehensive picture on how T cells and their derived cytokines could regulate memory retrieval and provide new insights into the nature of neuroimmune interactions at the transcriptional level in neurons. Overall, restoration of T cells or IL-4R signaling might lead to selective rescue of memory retrieval, and proffer a valuable strategy for treating memory loss.
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Overall design |
This experiment aims to compare the transcriptional profile of nuclei from 2 genotypes (Gad2cre+::IL-4Rfl/fl/Gad2cre-::IL-4Rfl/fl) and 2 activation states (engram/nonengram). All nuclei were collected from the dentate gyrus of mice after fear conditioning. All mice were housed under standard conditions and injected with labelled viruses to detect the activation state.
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Contributor(s) |
Herz J, Fu Z, Dykstra T, Kipnis J |
Citation(s) |
34793707 |
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Submission date |
Jan 06, 2020 |
Last update date |
May 12, 2023 |
Contact name |
Jonathan Kipnis |
Organization name |
Washington University in Saint Louis
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Department |
Pathology and Immunology
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Lab |
Kipnis Lab
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Street address |
4515 McKinley Ave
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City |
Saint Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE143183 |
GABAergic neuronal IL-4R mediates T cell effect on memory |
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Relations |
BioProject |
PRJNA599155 |
SRA |
SRP239568 |