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Series GSE143180 Query DataSets for GSE143180
Status Public on Oct 12, 2021
Title GABAergic neuronal IL-4R mediates T cell effect on memory [Il4raFlox]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The immune and nervous systems are highly interactive, however, little is known about how immune cells and their derived molecules impact brain function. Here we show that T cells play a critical role in retrieval of contextual fear conditioning (CFC) memory. Single-nuclei sequencing (snRNAseq) of engram neurons and non-engram neurons from the dentate gyrus one day after CFC learning revealed new evidence about differential expression of immune-related and synaptic plasticity-related genes, in SCID vs. wild-type mice. These findings present a comprehensive picture on how T cells and their derived cytokines could regulate memory retrieval and provide new insights into the nature of neuroimmune interactions at the transcriptional level in neurons. Overall, restoration of T cells or IL-4R signaling might lead to selective rescue of memory retrieval, and proffer a valuable strategy for treating memory loss.
 
Overall design This experiment aims to compare the transcriptional profile of nuclei from 2 genotypes (Gad2cre+::IL-4Rfl/fl/Gad2cre-::IL-4Rfl/fl) and 2 activation states (engram/nonengram). All nuclei were collected from the dentate gyrus of mice after fear conditioning. All mice were housed under standard conditions and injected with labelled viruses to detect the activation state.
 
Contributor(s) Herz J, Fu Z, Dykstra T, Kipnis J
Citation(s) 34793707
Submission date Jan 06, 2020
Last update date May 12, 2023
Contact name Jonathan Kipnis
Organization name Washington University in Saint Louis
Department Pathology and Immunology
Lab Kipnis Lab
Street address 4515 McKinley Ave
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM4251673 DG_Cre.Neg_Nonengram
GSM4251674 DG_Cre.Neg_Engram
GSM4251677 DG_Cre.Pos_Nonengram
This SubSeries is part of SuperSeries:
GSE143183 GABAergic neuronal IL-4R mediates T cell effect on memory
Relations
BioProject PRJNA599155
SRA SRP239568

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143180_RAW.tar 123.4 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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