|
| Status |
Public on Jan 04, 2020 |
| Title |
Investigate the role of MLKL in regulating TNF-induced endothelial cell inflammatory response |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Analyze the potential signaling pathways regulated by MLKL under TNF-induced endothelial cell inflammatory response
Methods: HUVECs were allowed to grow to confluency and were starved with basal medium containing 0.5% FBS for 6h. Then treat HUVECs with vehicle, NSA, TNF-alpha, TNF-alpha+NSA for 6h and extract RNA.
Results: NSA reduces the mRNA level of TNF-induced inflammation-related genes and most of these genes are downstream of NF-kappa B signaling pathway.
|
| |
|
| Overall design |
mRNA profiles of HUVECs treated with vehicle, NSA, TNF-alpha, TNF-alpha+NSA were generated by sequencing.
|
| |
|
| Contributor(s) |
Jialin D |
| Citation(s) |
32332696 |
| |
| Submission date |
Jan 03, 2020 |
| Last update date |
May 07, 2020 |
| Contact name |
Dai Jialin |
| E-mail(s) |
daijialin@sioc.ac.cn
|
| Phone |
15021859671
|
| Organization name |
Interdisciplinary Research Center of Biology and Chemistry
|
| Street address |
Room 507-1, Build 6, QiuYue Rd 26, PuDong District
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA598832 |
| SRA |
SRP239386 |
Less...
More...