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Series GSE14256 Query DataSets for GSE14256
Status Public on Mar 16, 2009
Title Human fibroblast stimulation with PDGF-BB or b-FGF
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We analyzed gene expression in human fibroblasts stimulated by platelet-derived growth factor-BB (PDGF-BB) or basic fibroblast growth factor (bFGF) for 1h and 24h. The results of two independent experiments were merged. SAM analysis identified 116 relevant probe sets. Hierarchical clustering of these probe sets showed divergent early gene regulation by PDGF and FGF but overlapping late response. We first analyzed genes commonly regulated by PDGF-BB and b-FGF more than 2 fold after 24h of stimulation and we found that these two growth factors repressed FOXO.
We then focused on the early gene expression response induced by both growth factors. We performed a fold change analysis and found 114 probe sets regulated by PDGF-BB and 42 probe sets regulated by b-FGF, 37 of which were shared between the two gene lists .
Keywords: Time course, cell Treatment comparison
Overall design Human foreskin fibroblasts (AG01518) were cultured (1.5E6 cells/10 cm dish) in MEM medium with 10% fetal calf serum and glutamine for 24h, then washed and incubated for 47h in serum-free medium. Cells were stimulated for 1h by PDGF-BB (25ng/ml) or b-FGF (10ng/ml, + heparine 50 microg/ml). Alternatively, cells were incubated for 24h in starvation medium and treated for 24h with growth factors, or left untreated for 48h in serum-free medium (control treatment).
Two biological replicates were performed
Contributor(s) Essaghir A, Demoulin J
Citation(s) 19244250, 20215436, 24762137
Submission date Dec 31, 2008
Last update date Dec 06, 2018
Contact name Jean-Baptiste Demoulin
Organization name Université catholique de Louvain
Department de Duve Institute
Street address av Hippocrate 75, B1.74.05
City Brussels
ZIP/Postal code B-1200
Country Belgium
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (10)
GSM356965 PDGF-BB stimulation for 24h_replicate1
GSM356966 PDGF-BB stimulation for 1h_replicate1
GSM356967 b-FGF stimulation for 24h_replicate1
BioProject PRJNA111211

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Supplementary file Size Download File type/resource
GSE14256_RAW.tar 22.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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