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| Status |
Public on Dec 17, 2019 |
| Title |
Early dynamics of photosynthetic Lhcf2 and Lhcf15 transcription and mRNA stabilities in response to herbivory-related decadienal in Phaeodactylum tricornutum |
| Organism |
Phaeodactylum tricornutum |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
During grazing, damaged cells of various diatom species release oxylipins whose functions include signaling to nearby undamaged cells. Our study focused on the effects of the oxylipin 2-trans, 4-trans-decadienal (DD) during the light cycle on photosynthetic light harvesting complex (LHC) mRNA regulation in Phaeodactylum tricornutum. RNAseq data revealed extensive suppression of LHC mRNAs measured at 3 h while a smaller set of LHC mRNAs were up-regulated. RNA metabolic labeling studies of divergently regulated Lhcf2 and Lhcf15 mRNAs documented dynamic shifts in their transcription and mRNA stabilities during the light cycle after DD treatment. The RNAseq dataset offers an extensive resource for expression profiling in the early response period to DD treatment.
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| Overall design |
mRNA profiles of Phaeodactylum tricornutum cells treated with 10 µM DD or DMSO solvent control for 3 and 6 h were generated from duplicate cell replicates by Illumina NextSeq platform (8 samples total).
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| Contributor(s) |
Islam S, Sabharwal T, Mehdy MC |
| Citation(s) |
32029835 |
| |
| Submission date |
Dec 16, 2019 |
| Last update date |
Feb 24, 2020 |
| Contact name |
Dennis Cates Wylie |
| Organization name |
University of Texas at Austin
|
| Department |
Center for Biomedical Research Support
|
| Street address |
102 East 24th Street
|
| City |
Austin |
| State/province |
Texas |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17128 |
Illumina HiSeq 2000 (Phaeodactylum tricornutum) |
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| Samples (8)
|
| GSM4221645 |
10 µM DD, 3 h, rep 2 |
| GSM4221646 |
0.1% DMSO, 6 h, rep 1 |
| GSM4221647 |
0.1% DMSO, 6 h, rep 2 |
| GSM4221648 |
10 µM DD, 6 h, rep 1 |
| GSM4221649 |
10 µM DD, 6 h, rep 2 |
|
| Relations |
| BioProject |
PRJNA595993 |
| SRA |
SRP237813 |
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