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Series GSE142137 Query DataSets for GSE142137
Status Public on Jul 27, 2021
Title A novel lncRNA XLOC_032768 rescues cisplatin-induced apoptosis of renal tubular cells and acute kidney injury by repressing TNF-α expression in trans-manner [mouse]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The cellular and molecular mechanisms by which cisplatin induces nephrotoxicity have been investigated extensively. However, the role of long non-coding RNAs (lncRNAs) in cisplatin-induced nephrotoxicity has not been received much attention. Here, we explore the functions and underlying mechanisms of a novel lncRNA XLOC_032768 in cisplatin-induced nephrotoxicity. Cisplatin treatment resulted in the apoptosis of renal epithelial cells in a mouse model and human renal proximal tubular epithelial cells (HK-2). By performing differentially expressed genes (DEGs) of the transcriptome data, we found the expression of lncRNA XLOC_032768 was significantly repressed by cisplatin treatment, which was also validated by RT-qPCR experiment of in vivo and in vitro model. Overexpression of lncRNA XLOC_032768 significantly inhibited the cisplatin-induced apoptosis of HK-2 and the expression of biomarkers for cisplatin-induced nephrotoxicity. Results from XLOC_032768 overexpression experiment revealed that XLOC_032768 target the tumor necrosis factor (TNF)-α in trans in HK-2 cells and mouse exposed to cisplatin. The administration of lncRNA XLOC_032768 attenuated renal dysfunction, morphological damage, and renal tubular cell injury, which was accompanied by TNF-α preservation, in a mouse model of cisplatin nephrotoxicity. These data indicate that XLOC_032768 suppressed cisplatin-induced apoptosis of tubular epithelial cells and acute kidney injury via a TNF mechanism. LncRNA XLOC_032768 would be a novel agent to reduce cisplatin-induced nephrotoxicity.
Overall design The C57 mouse for separated into two groups. The two groups were injected with either physiological saline (control) or cisplatin, respectively. The mouse kindey from the two group were extracted and prepared for the following experiments with two biological replicates.
Contributor(s) Zhou X, Cheng F
Citation(s) 32278129, 35059728
Submission date Dec 16, 2019
Last update date Feb 08, 2022
Contact name Dong Chen
Organization name ABLife, Inc.
Department Center for Genome Analysis
Street address 388 GaoXin 2nd Road, East Lake Hi-Tech Development Zone
City Wuhan
State/province Hubei
ZIP/Postal code 430075
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM4221381 CIS_1
GSM4221382 CIS_2
GSM4221383 con_1
This SubSeries is part of SuperSeries:
GSE142138 A novel lncRNA XLOC_032768 rescues cisplatin-induced apoptosis of renal tubular cells and acute kidney injury by repressing TNF-α expression in trans-manner
BioProject PRJNA595964
SRA SRP237798

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Supplementary file Size Download File type/resource
GSE142137_expressed_gene_FPKM.txt.gz 848.3 Kb (ftp)(http) TXT
GSE142137_expressed_gene_reads.txt.gz 812.1 Kb (ftp)(http) TXT
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Processed data are available on Series record

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