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| Status |
Public on Dec 01, 2020 |
| Title |
Differential gene expression in Hep3B and Hep3B SOX4-/- cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Overexpression of SOX4 in various kinds of cancers specimen was associated with poor prognosis of patients; however, the role of SOX4 in angiogenesis or tumor microenvironment modulation remains unclear. Therefore the endogenous SOX4 was knockout and the differential gene expression between Hep3B and Hep3B SOX4-/- cells were examined via genechip.
We found that the differentially expressed genes, EzH2, a SOX4-associated partner, and CXCL12, were repressed in Hep3B SOX4-/- cells compared with parental Hep3B; these results were further assessed via qRT-PCR in Hep3B SOX4-/- versus Hep3B cells.
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| Overall design |
Endogenous SOX4 knockout in Hep3B cells via the CRISPR/cas9 system that was designed as Hep3B SOX4-/-, the transcriptome of Hep3B and Hep3B SOX4-/- cells were analyzed via an Affymetrix GeneChip™ Human Transcriptome Array 2.0.
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| Contributor(s) |
Tsai C, Yu M |
| Citation missing |
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| Submission date |
Dec 12, 2019 |
| Last update date |
Dec 04, 2020 |
| Contact name |
Yun-Shien Lee |
| E-mail(s) |
bojack@mail.mcu.edu.tw
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| Organization name |
Ming Chuan Univ
|
| Department |
Dept. Biotechnology
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| Lab |
GMRCL
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| Street address |
TauYau
|
| City |
TauYau |
| State/province |
Taiwan |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA595153 |
