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Series GSE141834 Query DataSets for GSE141834
Status Public on Mar 26, 2020
Title Transcriptional Heterogeneity within the Glucocorticoid Response
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Steroid hormone receptors such as the Glucocorticoid Receptor (GR) mediate the transcriptional response to hormones and are frequently targeted in the treatment of human diseases. Experiments using bulk populations of cells have provided a detailed picture of the global transcriptional hormone response but are unable to interrogate cell-to-cell transcriptional heterogeneity. To examine the glucocorticoid response in individual cells, we performed single cell RNA sequencing (scRNAseq). The transcriptional response to hormone was robustly detected in individual cells and scRNAseq provided additional statistical power to identify over 100 GR-regulated genes that were not detected in bulk RNAseq. scRNAseq also revealed a large degree of cell-to-cell variability in the hormone response. On average, individual hormone-treated cells showed a response at only 30% of the total set of GR target genes. Understanding the basis of this heterogeneity will be critical for the development of more precise models of steroid hormone signaling.
Overall design Single-cell RNAseq and bulk total RNAseq in A1-2 human breast cancer cells
Web link
Contributor(s) Hoffman JA, Papas BN, Trotter KW, Archer TK
Citation(s) 32170217
Submission date Dec 11, 2019
Last update date Mar 29, 2020
Contact name Jackson Andrew Hoffman
Organization name NIEHS
Department DIR
Street address 111 TW Alexander Dr, Building 101 Room C432
City Research Triangle Park
State/province North Carolina
ZIP/Postal code 27709
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (26)
GSM4213967 A1-2 cells, EtOH_rep1, Bulk RNAseq
GSM4213968 A1-2 cells, EtOH_rep2, Bulk RNAseq
GSM4213969 A1-2 cells, EtOH_rep3, Bulk RNAseq
BioProject PRJNA594939
SRA SRP236872

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141834_RAW.tar 5.6 Gb (http)(custom) TAR (of BW)
GSE141834_bulkRNAseq_normalized_counts.txt.gz 4.5 Mb (ftp)(http) TXT
GSE141834_scRNAseq_rawCounts.txt.gz 12.8 Mb (ftp)(http) TXT
GSE141834_scRNAseq_seuratV3_normalized.txt.gz 121.9 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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