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Series GSE141708 Query DataSets for GSE141708
Status Public on Aug 07, 2020
Title mRNA profiles of human Dedifferentiated Adipose Cells and Adipose-derived stem cells in young donors
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Dedifferentiated Fat Cells (DAs) and Adipose-derived stem cells (ADSCs) are two main stem cells derived from adipose tissue. In order to explore whether there is difference in transcriptome between the two cells after multiple subcultures, and which kind of cell should be selected for different regenerative aim, high-throughput RNAseq were conducted to find differentially expressed genes. The subcutaneous adipose tissue of three young women (23-30 years old)patients with normal BMI (19-24) were obtained during liposuction operation on abdomen or thigh. Stromal Vascular Fraction (SVF) and adipocytes were obtained by collagenase digestion, ADSCs was cultured regularly, and DAs were cultured by a modified ceiling culture method. Only 186 genes with different expression between the two groups were obtained.The downregulated gene number of DAs vs. ADSCs was 112 and the upregulated number was 74. Many of the differentially expressed genes are involved in the biological functions such as transcription regulator, protein translation regulation, cytokine interaction and energy metabolism regulation. These data may provide a foundation for further clinical administration of stem cells derived from adipose tissues.
Overall design Comparison of mRNA expression profiles between human Dedifferentiated Adipose Cells and Adipose-derived stem cells obtained from tumenecent liposuction in young donors
Contributor(s) Nie F, Bi H
Citation(s) 33200799
Submission date Dec 09, 2019
Last update date Dec 08, 2020
Contact name Fangfei Nie
Organization name Peking university third hospital
Department Department of Plastic Surgery
Street address 49 North Garden Rd.,Haidian District
City Beijing
ZIP/Postal code 100191
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4211341 ADSCs-1
GSM4211342 ADSCs-2
GSM4211343 ADSCs-3
BioProject PRJNA594410
SRA SRP235376

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Supplementary file Size Download File type/resource
GSE141708_fpkm.txt.gz 613.6 Kb (ftp)(http) TXT
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Processed data are available on Series record

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