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| Status |
Public on Feb 09, 2020 |
| Title |
The role of histone H3K27 acetylation for enhancer activity in embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
By substituting lysine 27 in histone variant H3.3 to arginine in mouse embryonic stem cells, we measured H3K27ac/H3K4me1/H3K4me3 and other acetylation modifications in H3.3K27R mutant cells, together with RNA-seq and ATAC-seq to measure the transcription activity and chromatin accessibility, respectively.
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| Overall design |
WT mouse ESC served as controls , and two H3.3K27R mutant lines (Mut18 and Mut43) were sequenced. Two independent biological replicates were measured for ChIP-seq / RNA-seq /ATAC-seq experiments, and input data were shared among histone ChIP experiments for each cell line.
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| Contributor(s) |
Zhang T, Zhang Z, Dong Q, Xiong J, Zhu B |
| Citation(s) |
32085783 |
| |
| Submission date |
Dec 05, 2019 |
| Last update date |
Mar 09, 2020 |
| Contact name |
Zhuqiang Zhang |
| E-mail(s) |
zhangzhuqiang@ibp.ac.cn
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| Organization name |
Institute of Biophysics, CAS
|
| Street address |
15 Datun Road, Chaoyang District.
|
| City |
Beijing |
| State/province |
--- |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (2) |
| GPL23479 |
BGISEQ-500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (69)
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| Relations |
| BioProject |
PRJNA593793 |
| SRA |
SRP234881 |
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