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| Status |
Public on Apr 29, 2020 |
| Title |
Transcriptome wide analysis of differential gene expression and polyadenylation site usages caused by inhibition of CDK12 and/or CDK13 in MV4;11 cells (3’ Quant-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used 3' RNA-Seq to determine the effects of analog senstive CDK12, CDK13 and dual inhibition on gene expression and polyadenatltion site usages, and to test the redundancy between CDK12 and CDK13 in maintaining global transcrition on the tramcrotpomte level.
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| Overall design |
we performed 3’RNA-seq (Quantseq) on WT, CDK12AS/NULL, CDK13AS/AS, #1CDK12AS/NULL;CDK13AS/NULL and #2CDK12AS/NULL;CDK13AS/AS MV4;11 clones treated with 1-NM-PP1 or vehicle for 4 hours. 6 hours THZ531 or vehicle treatments were applied on parental MV4;11 cells
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| Contributor(s) |
Fan Z, Devlin JR, Vervoort SJ, Johnstone RW |
| Citation(s) |
32917631 |
| |
| Submission date |
Dec 03, 2019 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Zheng Fan |
| E-mail(s) |
zheng.fan@petermac.org
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
305 Grattan Street
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3000 |
| Country |
Australia |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA593219 |
| SRA |
SRP234546 |
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